Journal: PLoS ONE

Article Title: Locating and Activating Molecular ‘Time Bombs’: Induction of Mycolata Prophages

doi: 10.1371/journal.pone.0159957

Figure Lengend Snippet: Summary of strains used in Mycolata prophage induction studies.

Article Snippet: Dietzia maris , CON27 , [ ] , Dmar27, DSMZ 43672 , 0 , NA , NA , - , -.

Techniques:

Journal: SpringerPlus

Article Title: Fulvestrant up regulates UGT1A4 and MRP s through ERα and c-Myb pathways: a possible primary drug disposition mechanism

doi: 10.1186/2193-1801-2-620

Figure Lengend Snippet: Up regulation of UGT1A4 mRNA expression in MCF7 and HepG2 cell lines treated with fulvestrant. (a) MCF7 and HepG2 cells ± transfection with ERα siRNA were treated with various concentrations of fulvestrant before UGT1A4 gene expression was measured. All data was normalized to β-actin. (b) MCF7 and HepG2 cells were transfected as in (A), and then all were treated with 10 nM fulvestrant and analyzed for UGT1A4 expression at various time points.

Article Snippet: ERα siRNA(h), c-Myb siRNA (h), ERα, c-Myb and UGT1A4 primary and secondary antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).

Techniques: Expressing, Transfection, Gene Expression

Journal: SpringerPlus

Article Title: Fulvestrant up regulates UGT1A4 and MRP s through ERα and c-Myb pathways: a possible primary drug disposition mechanism

doi: 10.1186/2193-1801-2-620

Figure Lengend Snippet: Correlation of UGT1A4, MRP and ERα in HepG2 and MCf7 Cell lines: Correlation analysis of MCF7/HepG2 UGT1A4 mRNA expression level with its own UGT1A4 protein, Anastrozole glucuronidation, ERα mRNA, ERα protein, MRP 1, MRP 2 and MRP 3

Article Snippet: ERα siRNA(h), c-Myb siRNA (h), ERα, c-Myb and UGT1A4 primary and secondary antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).

Techniques: Expressing

Journal: SpringerPlus

Article Title: Fulvestrant up regulates UGT1A4 and MRP s through ERα and c-Myb pathways: a possible primary drug disposition mechanism

doi: 10.1186/2193-1801-2-620

Figure Lengend Snippet: Correlation of UGT1A4 mRNA with ER expression. Correlation of UGT1A4 mRNA with ER protein (a) and with ER mRNA (b) in HepG2 and MCF7 cell lines treated with fulvestrant. Cells were treated with 10 nM fulvestrant. UGT1A4 and ER mRNA and protein expressions were measured at various time points.

Article Snippet: ERα siRNA(h), c-Myb siRNA (h), ERα, c-Myb and UGT1A4 primary and secondary antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).

Techniques: Expressing

Journal: SpringerPlus

Article Title: Fulvestrant up regulates UGT1A4 and MRP s through ERα and c-Myb pathways: a possible primary drug disposition mechanism

doi: 10.1186/2193-1801-2-620

Figure Lengend Snippet: UGT1A4 luciferase activity measured in MCF7 and HepG2 cell lines. Cells were transfected either with empty vector or UGT1A4 reference promoter. Then treated with 10 mM fulvestrant and luciferase activity was measured *p value < 0.01.

Article Snippet: ERα siRNA(h), c-Myb siRNA (h), ERα, c-Myb and UGT1A4 primary and secondary antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).

Techniques: Luciferase, Activity Assay, Transfection, Plasmid Preparation

Journal: SpringerPlus

Article Title: Fulvestrant up regulates UGT1A4 and MRP s through ERα and c-Myb pathways: a possible primary drug disposition mechanism

doi: 10.1186/2193-1801-2-620

Figure Lengend Snippet: Promoter variant luciferase activity in various cell lines . Promoter variant luciferase activity measured in MCF7 (a) and HepG2 (b) cells. Cells were transfected either with UGT1A4 common allele promoter or with promoters with single variants at -163, -217, and -219 and with a promoter with all three variant (complete variant). Cells were then treated with 10 mM fulvestrant and luciferase activity was measured, and is displayed as percentage difference * p value < 0.01, Δ p value ≤ 0.01.

Article Snippet: ERα siRNA(h), c-Myb siRNA (h), ERα, c-Myb and UGT1A4 primary and secondary antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).

Techniques: Variant Assay, Luciferase, Activity Assay, Transfection

Journal: SpringerPlus

Article Title: Fulvestrant up regulates UGT1A4 and MRP s through ERα and c-Myb pathways: a possible primary drug disposition mechanism

doi: 10.1186/2193-1801-2-620

Figure Lengend Snippet: Luciferase activity in MCF7 cells +/- c-Myb transfected with UGT1A4 common or variant promoters. Cells were transfected either with UGT1A4 promoter constructs containing all common alleles or UGT1A4 promoter constructs containing all variant alleles in locations -163, -217 and -219. Cells were treated with 10 mM fulvestrant and luciferase activity was measured, and is displayed as fold-change *p value = 0.01.

Article Snippet: ERα siRNA(h), c-Myb siRNA (h), ERα, c-Myb and UGT1A4 primary and secondary antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).

Techniques: Luciferase, Activity Assay, Transfection, Variant Assay, Construct